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PET TRACERS AVAILABLE AT CMGI
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[18F]Fluoride
Uses: [18F]Fluoride in aqueous solution is used primarily to image bone and therefore can provide information on features of the skeleton.
Half life: 110 minutes
Availability: daily
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[18F]FDG
Chemical name: [18F]Fluorodeoxyglucose
Uses: FDG, one of the most commonly utilized tracers for PET studies, is used to measure tissue glucose metabolism. It has important diagnostic and research applications in oncology, neurology, and cardiology.
Half life: 110 minutes
Availability: daily
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[18F]FHBG
Chemical name: 9-(4-[18F]Fluoro-3-Hydroxymethylbutyl) Guanine
Uses: Imaging probe for the herpes simplex virus type 1 thymidine kinase (HSV1-tk) reporter gene, which can be transfected into malignant cells in experimental suicide gene therapy.
Half life: 110 minutes
Availability: daily
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[18F]FLT
Chemical name: [18F]Fluorothymidine
Uses: A marker for cell proliferation that is more specific for tumor imaging than [18F]FDG.
Half life: 110 minutes
Availability: daily
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[18F]FMISO
Chemical name: [18F]Fluoromisonidazole
Uses: Used for the detection of hypoxia in tumors, myocardial infarcts, or cerebral ischemia.
Half life: 110 minutes
Availability: daily
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[64Cu]Cu-PTSM
Chemical name: [64Cu]Cu-pyruvaldehyde-bis[N4-methylthiosemicarbazone]
Uses: Tracer for blood flow, hypoxia, and cell binding studies.
Half life: 12.7 hours
Availability: 64Cu is available weekly. Please inquire for specific dates.
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[64Cu]Cu-Chelate-Conjugates (above)
Uses: Molecular probes (e.g., antibodies, peptides, and nanoparticles) can be conjugated to chelating (metal binding) agents, which bind 64Cu. The example structure is a protein conjugated to the 64Cu binding chelator tetraazacyclotetradecane (TETA).
Half life: 12.7 hours
Availability: 64Cu is available weekly. Please inquire for specific dates.
Special notes: CMGI Radiochemistry can conjugate a chelating agent to a client-supplied molecular probe as a custom synthesis; or the client may provide a chelate-conjugated probe.
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Facility Design and Equipment
The Radiochemistry facility is equipped to produce radionuclides and prepare PET tracers by automated and manual synthesis.
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An 11 MeV cyclotron (Siemens RDS111) is used to produce 18F, 11C, 13N, and 15O.
- Radionuclides are delivered directly to shielded hot cells (above), in which radiochemical reactions are performed. The hot cells are shielded with 3 inches of lead and 9 inch leaded glass windows.
- Two hot cells are equipped with remote manipulators, to perform manual reactions.
- Automated synthesis units (left) for 18F and 11C chemistry are used for routine syntheses.
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Click the image below to see radiosynthesis performed via remote manipulators
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Novel Radiochemistry and Custom Synthesis
4-[18F]fluorobenzoic acid (FBA) and 2-[18F]fluoropropionic acid (FPA) are used as building blocks for the synthesis of complex molecules such as [18F]-fluoropaclitaxel and as prosthetic groups for the labeling of peptides and proteins. The synthesis of FBA has been automated.
Peptides may be site-specifically radiolabeled in the solid phase with 18F via the prosthetic groups 4-[18F]fluorobenzoic acid (FBA) or 2-[18F]fluoropropionic acid (FPA). In cases where solution-phase labeling is preferred, Cu catalyzed 1,3-dipolar Huisgen cycloaddition (“Click” chemistry) is used. Peptides may also be conjugated to a chelating (metal binding) agent for 64Cu radiolabeling.
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“Click” chemistry for radiolabeling of
peptides with [18F]fluoroalkynes.
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Solid phase peptide labeling using
4-[18F]fluorobenzoic acid (FBA).
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